第六十四条 船舶擅自进入、停靠国家禁止、限制进入的水域或者岛屿的，对船舶负责人及有关责任人员处一千元以上二千元以下罚款；情节严重的，处五日以下拘留，可以并处二千元以下罚款。
This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
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